| 1. Round | 10/08/2024 | NEC Nijmegen | - | FC Twente | 1:2 | ||||
| 2. Round | 17/08/2024 | FC Twente | - | Sparta Rotterdam | 1:1 | ||||
| 5. Round | 14/09/2024 | FC Twente | - | PEC Zwolle | 1:1 | ||||
| 3. Round | 17/09/2024 | FC Twente | - | sc Heerenveen | 2:0 | ||||
| 6. Round | 22/09/2024 | Almere City FC | - | FC Twente | 0:5 |  |
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| 7. Round | 29/09/2024 | FC Twente | - | NAC Breda | 1:0 | |
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| 8. Round | 06/10/2024 | Feyenoord | - | FC Twente | 2:1 | ||||
| 9. Round | 19/10/2024 | RKC Waalwijk | - | FC Twente | 2:2 |  |
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| 10. Round | 27/10/2024 | FC Twente | - | Heracles Almelo | 5:0 | ||||
| 11. Round | 02/11/2024 | Willem II | - | FC Twente | 0:1 | ||||
| 12. Round | 10/11/2024 | FC Twente | - | AFC Ajax | 2:2 | ||||
| 13. Round | 23/11/2024 | Fortuna Sittard | - | FC Twente | 1:2 | ||||
| 14. Round | 01/12/2024 | FC Twente | - | Go Ahead Eagles | 3:2 | ||||
| 15. Round | 06/12/2024 | PSV Eindhoven | - | FC Twente | 6:1 | ||||
| 16. Round | 15/12/2024 | FC Twente | - | FC Groningen | 2:0 | ||||
| 18. Round | 12/01/2025 | FC Twente | - | Willem II | 6:2 | |
Netherlands