| 2. Round | 16/03/2025 | Birmingham Legion | - | Louisville City FC | 1:1 | ||||
| 4. Round | 29/03/2025 | Louisville City FC | - | Detroit City FC | 2:0 | ||||
| 7. Round | 20/04/2025 | Tampa Bay Rowdies | - | Louisville City FC | 1:2 | ||||
| 9. Round | 11/05/2025 | Louisville City FC | - | Pittsburgh Riverhounds | 0:0 | ||||
| 11. Round | 25/05/2025 | Monterey Bay FC | - | Louisville City FC | 0:2 |
USA