| 17. Round | 18/02/2001 | Sigma Olomouc | - | Chmel Blsany | 3:0 | ||||
| 18. Round | 04/03/2001 | FC Marila Příbram | - | Chmel Blsany | 3:0 | ||||
| 19. Round | 11/03/2001 | Chmel Blsany | - | Viktoria Plzeň | 2:1 | ||||
| 20. Round | 18/03/2001 | FC Stavo Artikel Brno | - | Chmel Blsany | 0:0 | ||||
| 21. Round | 31/03/2001 | Chmel Blsany | - | CU Bohemians Praha | 0:0 | ||||
| 22. Round | 04/04/2001 | Chmel Blsany | - | 1. FC Synot Stare Mesto | 2:2 | ||||
| 23. Round | 08/04/2001 | Viktoria Žižkov | - | Chmel Blsany | 1:0 | ||||
| 24. Round | 15/04/2001 | Chmel Blsany | - | Slovan Liberec | 1:0 | ||||
| 25. Round | 21/04/2001 | FK Jablonec 97 | - | Chmel Blsany | 0:2 | ||||
| 26. Round | 29/04/2001 | Chmel Blsany | - | Baník Ostrava | 3:0 | ||||
| 27. Round | 07/05/2001 | Dynamo České Budějovice | - | Chmel Blsany | 1:1 |  |
|||
| 28. Round | 13/05/2001 | Chmel Blsany | - | AC Sparta Praha | 2:2 | ||||
| 29. Round | 18/05/2001 | Slavia Praha | - | Chmel Blsany | 2:3 | |
 |
||
| 30. Round | 25/05/2001 | Chmel Blsany | - | FK Teplice | 3:1 | |
Czech Republic