| 1. Round | 15/09/2000 | Olympiakos Piraeus | - | Ethnikos Asteras | 6:0 | ||||
| 9. Round | 02/12/2000 | Ethnikos Asteras | - | OFI Heraklion | 1:0 | ||||
| 12. Round | 03/01/2001 | PAOK Saloniki | - | Ethnikos Asteras | 2:1 | ||||
| 14. Round | 13/01/2001 | Iraklis Saloniki | - | Ethnikos Asteras | 4:0 |  |
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| 16. Round | 27/01/2001 | Ethnikos Asteras | - | Olympiakos Piraeus | 0:1 | ||||
| 17. Round | 03/02/2001 | Ionikos Nikea | - | Ethnikos Asteras | 2:1 | |
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| 19. Round | 17/02/2001 | Panachaiki GE | - | Ethnikos Asteras | 1:0 | ||||
| 20. Round | 24/02/2001 | Ethnikos Asteras | - | PS Kalamata | 1:3 | |
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| 23. Round | 17/03/2001 | Aris Saloniki | - | Ethnikos Asteras | 2:0 | ||||
| 24. Round | 31/03/2001 | OFI Heraklion | - | Ethnikos Asteras | 2:2 | |
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| 25. Round | 07/04/2001 | Ethnikos Asteras | - | Paniliakos Pyrgos | 1:1 | ||||
| 26. Round | 21/04/2001 | Panathinaikos | - | Ethnikos Asteras | 2:0 | ||||
| 27. Round | 28/04/2001 | Ethnikos Asteras | - | PAOK Saloniki | 3:3 | |
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| 28. Round | 05/05/2001 | Skoda Xanthi | - | Ethnikos Asteras | 3:1 | ||||
| 30. Round | 26/05/2001 | Athinaikos | - | Ethnikos Asteras | 1:3 |
Greece