| 1. Round | 13/03/2021 | FK Pohronie | - | FK Senica | 3:0 | ||||
| 2. Round | 20/03/2021 | FK Senica | - | ŠKF Sereď | 1:0 | ||||
| 3. Round | 03/04/2021 | MFK Zemplín Michalovce | - | FK Senica | 1:0 | ||||
| 4. Round | 10/04/2021 | FK Senica | - | MFK Ružomberok | 0:1 | ||||
| 5. Round | 17/04/2021 | FC Nitra | - | FK Senica | 0:3 | ||||
| 6. Round | 24/04/2021 | FK Senica | - | MFK Zemplín Michalovce | 1:1 | ||||
| 7. Round | 01/05/2021 | FK Senica | - | FC Nitra | 1:0 | ||||
| 8. Round | 08/05/2021 | MFK Ružomberok | - | FK Senica | 1:1 | ||||
| 9. Round | 15/05/2021 | FK Senica | - | FK Pohronie | 0:0 | ||||
| 10. Round | 22/05/2021 | ŠKF Sereď | - | FK Senica | 4:1 |
Czech Republic