| 1. Round | 08/08/2020 | FK Senica | - | MŠK Žilina | 0:4 | ||||
| 2. Round | 12/08/2020 | ŠKF Sereď | - | FK Senica | 4:0 | ||||
| 3. Round | 15/08/2020 | FK Senica | - | MFK Zemplín Michalovce | 3:0 | ||||
| 5. Round | 29/08/2020 | FK Senica | - | FC Nitra | 0:0 | ||||
| 6. Round | 11/09/2020 | Zlaté Moravce | - | FK Senica | 2:2 | ||||
| 7. Round | 20/09/2020 | FK Senica | - | FC DAC 1904 | 2:4 | ||||
| 8. Round | 26/09/2020 | FK Senica | - | Spartak Trnava | 0:2 | ||||
| 4. Round | 30/09/2020 | Slovan Bratislava | - | FK Senica | 2:0 | ||||
| 13. Round | 17/11/2020 | FK Senica | - | ŠKF Sereď | 2:2 | ||||
| 14. Round | 21/11/2020 | MFK Zemplín Michalovce | - | FK Senica | 0:1 | ||||
| 15. Round | 29/11/2020 | FK Senica | - | Slovan Bratislava | 0:3 | ||||
| 16. Round | 05/12/2020 | FC Nitra | - | FK Senica | 1:1 | ||||
| 17. Round | 12/12/2020 | FK Senica | - | Zlaté Moravce | 1:1 | ||||
| 18. Round | 15/12/2020 | FC DAC 1904 | - | FK Senica | 2:0 | ||||
| 20. Round | 20/02/2021 | FK Senica | - | AS Trenčín | 2:3 | ||||
| 21. Round | 27/02/2021 | MFK Ružomberok | - | FK Senica | 2:3 | ||||
| 22. Round | 06/03/2021 | FK Senica | - | FK Pohronie | 1:1 |
Czech Republic