| 1. Round | 23/02/2025 | Seattle Sounders | - | Charlotte FC | 2:2 | ||||
| 2. Round | 01/03/2025 | Charlotte FC | - | Atlanta United FC | 2:0 | ||||
| 3. Round | 09/03/2025 | Inter Miami CF | - | Charlotte FC | 1:0 | ||||
| 4. Round | 15/03/2025 | Charlotte FC | - | FC Cincinnati | 2:0 | ||||
| 6. Round | 29/03/2025 | Colorado Rapids | - | Charlotte FC | 2:0 | ||||
| 7. Round | 05/04/2025 | Charlotte FC | - | Nashville SC | 2:1 | ||||
| 8. Round | 13/04/2025 | CF Montréal | - | Charlotte FC | 0:1 | ||||
| 9. Round | 20/04/2025 | Charlotte FC | - | San Diego FC | 3:0 |  |
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| 10. Round | 27/04/2025 | Charlotte FC | - | New England Revolution | 0:1 | ||||
| 12. Round | 11/05/2025 | Nashville SC | - | Charlotte FC | 2:1 | ||||
| 13. Round | 15/05/2025 | Orlando City | - | Charlotte FC | 3:1 | ||||
| 14. Round | 18/05/2025 | Charlotte FC | - | Chicago Fire | 1:4 | ||||
| 16. Round | 29/05/2025 | New York RB | - | Charlotte FC | 4:2 | ||||
| 17. Round | 01/06/2025 | Toronto FC | - | Charlotte FC | 0:2 |
USA