| 2. Round | 16/03/2024 | El Paso Locomotive | - | Louisville City FC | 0:1 | ||||
| 3. Round | 23/03/2024 | Louisville City FC | - | Pittsburgh Riverhounds | 3:1 | ||||
| 4. Round | 30/03/2024 | Louisville City FC | - | Birmingham Legion | 5:0 | ||||
| 15. Round | 16/06/2024 | Tampa Bay Rowdies | - | Louisville City FC | 3:2 | ||||
| 18. Round | 07/07/2024 | Oakland Roots | - | Louisville City FC | 1:0 | ||||
| 20. Round | 20/07/2024 | Louisville City FC | - | Colorado Springs Switchbacks | 2:1 | ||||
| 25. Round | 25/08/2024 | Hartford Athletic | - | Louisville City FC | 2:1 | ||||
| 34. Round | 20/10/2024 | San Antonio FC | - | Louisville City FC | 1:1 |
USA