| 2. Round | 02/05/2021 | Rio Grande Valley FC | - | New Mexico United | 1:0 |  |
|||
| 2. Round | 07/05/2021 | Rio Grande Valley FC | - | San Diego Loyal | 1:0 | ||||
| 2. Round | 17/05/2021 | Rio Grande Valley FC | - | San Antonio FC | 2:1 | |
|||
| 2. Round | 23/05/2021 | El Paso Locomotive | - | Rio Grande Valley FC | 1:0 | ||||
| 4. Round | 01/08/2021 | Phoenix Rising | - | Rio Grande Valley FC | 3:1 | ||||
| 5. Round | 05/08/2021 | Real Monarchs SLC | - | Rio Grande Valley FC | 0:3 | ||||
| 5. Round | 09/08/2021 | Rio Grande Valley FC | - | San Antonio FC | 1:2 | |
|||
| 5. Round | 18/08/2021 | Austin Bold | - | Rio Grande Valley FC | 1:1 | ||||
| 6. Round | 12/09/2021 | Colorado Springs Switchbacks | - | Rio Grande Valley FC | 1:1 |
USA