| 1. Round | 14/08/2016 | Liverpool FC U23 | - | Southampton FC U23 | 1:4 |  |
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| 3. Round | 28/08/2016 | Sunderland AFC U23 | - | Southampton FC U23 | 3:0 | ||||
| 5. Round | 19/09/2016 | Southampton FC U23 | - | Leicester City U23 | 2:2 | ||||
| 6. Round | 23/09/2016 | Southampton FC U23 | - | Tottenham Hotspur U23 | 1:0 |  |
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| 8. Round | 24/10/2016 | Southampton FC U23 | - | Manchester City U23 | 0:3 | ||||
| 9. Round | 31/10/2016 | Derby County U23 | - | Southampton FC U23 | 2:0 | ||||
| 10. Round | 21/11/2016 | Chelsea FC U23 | - | Southampton FC U23 | 3:2 | ||||
| 11. Round | 28/11/2016 | Southampton FC U23 | - | Everton FC U23 | 1:1 | ||||
| 12. Round | 09/12/2016 | Southampton FC U23 | - | Sunderland AFC U23 | 0:2 | ||||
| 14. Round | 06/01/2017 | Reading FC U23 | - | Southampton FC U23 | 4:2 | ||||
| 16. Round | 30/01/2017 | Tottenham Hotspur U23 | - | Southampton FC U23 | 3:1 | ||||
| 17. Round | 06/02/2017 | Leicester City U23 | - | Southampton FC U23 | 0:2 |
England