| 1. Round | 22/08/1965 | AFC Ajax | - | DOS Utrecht | 2:1 | ||||
| 2. Round | 29/08/1965 | MVV | - | AFC Ajax | 0:1 | ||||
| 3. Round | 05/09/1965 | AFC Ajax | - | PSV Eindhoven | 2:0 |  |
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| 4. Round | 12/09/1965 | Telstar | - | AFC Ajax | 1:2 | |
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| 5. Round | 26/09/1965 | AFC Ajax | - | Go Ahead | 4:0 | |
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| 6. Round | 03/10/1965 | AVC Heracles | - | AFC Ajax | 1:1 | ||||
| 7. Round | 10/10/1965 | AFC Ajax | - | RC&FC Sparta | 0:1 | ||||
| 8. Round | 24/10/1965 | DWS Amsterdam | - | AFC Ajax | 0:2 | ||||
| 9. Round | 31/10/1965 | AFC Ajax | - | Fortuna '54 | 5:1 | |
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| 10. Round | 28/11/1965 | ADO | - | AFC Ajax | 2:3 | |
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| 11. Round | 05/12/1965 | AFC Ajax | - | GVAV Groningen | 5:1 | ||||
| 12. Round | 12/12/1965 | Willem II | - | AFC Ajax | 1:4 | ||||
| 17. Round | 27/02/1966 | AFC Ajax | - | MVV | 1:1 | ||||
| 21. Round | 27/03/1966 | AFC Ajax | - | AVC Heracles | 1:0 | ||||
| 30. Round | 22/05/1966 | USV Elinkwijk | - | AFC Ajax | 3:3 |
Netherlands