| 22. Round | 20/01/2024 | Calcio Padova | - | US Alessandria 1912 | 1:0 | ||||
| 23. Round | 27/01/2024 | US Alessandria 1912 | - | Arzignano Valchiampo | 0:0 | ||||
| 24. Round | 04/02/2024 | FC Lumezzane | - | US Alessandria 1912 | 2:1 |  |
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| 26. Round | 13/02/2024 | US Alessandria 1912 | - | Mantova 1911 SSD | 0:1 | |
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| 27. Round | 18/02/2024 | Atalanta II | - | US Alessandria 1912 | 2:0 | |
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| 25. Round | 21/02/2024 | AC Pro Sesto | - | US Alessandria 1912 | 1:2 | ||||
| 28. Round | 24/02/2024 | US Alessandria 1912 | - | UC AlbinoLeffe | 0:1 | |
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| 29. Round | 01/03/2024 | US Pergolettese | - | US Alessandria 1912 | 0:1 | ||||
| 30. Round | 05/03/2024 | US Alessandria 1912 | - | Trento Calcio 1921 | 0:1 | |
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| 33. Round | 23/03/2024 | Giana Erminio | - | US Alessandria 1912 | 3:0 | ||||
| 31. Round | 26/03/2024 | US Triestina | - | US Alessandria 1912 | 3:1 | |
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| 34. Round | 30/03/2024 | US Alessandria 1912 | - | Pro Vercelli | 1:1 |  |
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| 35. Round | 06/04/2024 | US Fiorenzuola | - | US Alessandria 1912 | 2:0 | ||||
| 37. Round | 20/04/2024 | FC Legnago Salus | - | US Alessandria 1912 | 0:0 | |
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| 38. Round | 28/04/2024 | US Alessandria 1912 | - | L.R. Vicenza | 1:2 |
Spain