| 1. Round | 23/02/2025 | Columbus Crew | - | Chicago Fire | 4:2 |  |
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| 2. Round | 02/03/2025 | New England Revolution | - | Columbus Crew | 0:1 | |
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| 3. Round | 08/03/2025 | Columbus Crew | - | Houston Dynamo | 0:0 | ||||
| 4. Round | 16/03/2025 | San Diego FC | - | Columbus Crew | 1:1 | ||||
| 5. Round | 22/03/2025 | Columbus Crew | - | New York City FC | 0:0 | ||||
| 6. Round | 29/03/2025 | D.C. United | - | Columbus Crew | 1:2 |  |
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| 7. Round | 06/04/2025 | Columbus Crew | - | CF Montréal | 2:1 | |
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| 8. Round | 14/04/2025 | St. Louis City SC | - | Columbus Crew | 1:2 | ||||
| 9. Round | 19/04/2025 | Columbus Crew | - | Inter Miami CF | 0:1 | ||||
| 10. Round | 27/04/2025 | Columbus Crew | - | San Jose Earthquakes | 2:1 | ||||
| 11. Round | 04/05/2025 | Columbus Crew | - | Charlotte FC | 4:2 | |
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| 12. Round | 11/05/2025 | Philadelphia Union | - | Columbus Crew | 2:2 | ||||
| 13. Round | 15/05/2025 | CF Montréal | - | Columbus Crew | 1:1 | |
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| 14. Round | 18/05/2025 | Columbus Crew | - | FC Cincinnati | 1:1 | ||||
| 15. Round | 25/05/2025 | Charlotte FC | - | Columbus Crew | 3:2 | ||||
| 16. Round | 29/05/2025 | Columbus Crew | - | Nashville SC | 2:2 | ||||
| 17. Round | 01/06/2025 | Inter Miami CF | - | Columbus Crew | 5:1 |
Canada
Jamaica 