| 22. Round | 31/01/2010 | VfL Osnabrück | - | Kickers Offenbach | 1:0 | ||||
| 23. Round | 06/02/2010 | FC Ingolstadt 04 | - | VfL Osnabrück | 0:0 | ||||
| 24. Round | 14/02/2010 | VfL Osnabrück | - | SpVgg Unterhaching | 1:0 | ||||
| 25. Round | 21/02/2010 | FC Carl Zeiss Jena | - | VfL Osnabrück | 1:1 | ||||
| 26. Round | 27/02/2010 | VfL Osnabrück | - | Dynamo Dresden | 1:1 | ||||
| 28. Round | 13/03/2010 | VfL Osnabrück | - | Wuppertaler SV | 1:1 | ||||
| 21. Round | 16/03/2010 | Werder Bremen II | - | VfL Osnabrück | 0:1 | ||||
| 29. Round | 19/03/2010 | VfB Stuttgart II | - | VfL Osnabrück | 0:1 | ||||
| 30. Round | 27/03/2010 | VfL Osnabrück | - | SV Wehen Wiesbaden | 0:0 | ||||
| 31. Round | 31/03/2010 | SV Sandhausen | - | VfL Osnabrück | 3:2 |  |
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| 32. Round | 03/04/2010 | VfL Osnabrück | - | Bayern München II | 4:1 | |
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| 33. Round | 10/04/2010 | Erzgebirge Aue | - | VfL Osnabrück | 3:0 | ||||
| 34. Round | 14/04/2010 | VfL Osnabrück | - | Rot-Weiß Erfurt | 3:1 | ||||
| 35. Round | 17/04/2010 | Jahn Regensburg | - | VfL Osnabrück | 2:2 | ||||
| 36. Round | 24/04/2010 | Borussia Dortmund II | - | VfL Osnabrück | 1:2 | ||||
| 37. Round | 30/04/2010 | VfL Osnabrück | - | Holstein Kiel | 3:1 | ||||
| 38. Round | 08/05/2010 | Wacker Burghausen | - | VfL Osnabrück | 0:1 |
Germany