| 8. Round | 18/09/2009 | VfR Aalen | - | Karlsruher SC II | 2:1 | ||||
| 9. Round | 26/09/2009 | Karlsruher SC II | - | 1. FC Nürnberg II | 0:2 | ||||
| 11. Round | 17/10/2009 | Karlsruher SC II | - | SV Wehen Wiesbaden II | 3:0 | ||||
| 12. Round | 23/10/2009 | SpVgg Weiden | - | Karlsruher SC II | 4:3 | ||||
| 17. Round | 05/12/2009 | Karlsruher SC II | - | SpVgg Greuther Fürth II | 2:0 | ||||
| 19. Round | 11/12/2009 | SC Freiburg II | - | Karlsruher SC II | 1:1 | ||||
| 18. Round | 19/12/2009 | Karlsruher SC II | - | SC Pfullendorf | 3:2 | ||||
| 20. Round | 28/02/2010 | Eintracht Frankfurt II | - | Karlsruher SC II | 1:2 | ||||
| 22. Round | 13/03/2010 | Bayern Alzenau | - | Karlsruher SC II | 0:0 | ||||
| 23. Round | 21/03/2010 | Karlsruher SC II | - | SSV Reutlingen | 3:0 | ||||
| 24. Round | 26/03/2010 | Hessen Kassel | - | Karlsruher SC II | 3:0 | ||||
| 28. Round | 24/04/2010 | SV Wehen Wiesbaden II | - | Karlsruher SC II | 1:2 | ||||
| 30. Round | 07/05/2010 | TSV 1860 München II | - | Karlsruher SC II | 3:2 |
France