| 2. Round | 22/08/2016 | Vitesse (J) | - | Jong FC Twente | 1:3 | ||||
| 3. Round | 28/08/2016 | Jong FC Twente | - | TEC VV | 2:3 | ||||
| 8. Round | 03/10/2016 | Sparta Rotterdam (J) | - | Jong FC Twente | 4:2 | ||||
| 9. Round | 07/10/2016 | Jong FC Twente | - | AZ Alkmaar (J) | 2:3 | ||||
| 11. Round | 29/10/2016 | Jong FC Twente | - | HHC Hardenberg | 0:1 | ||||
| 15. Round | 27/11/2016 | De Treffers | - | Jong FC Twente | 2:1 | ||||
| 25. Round | 05/03/2017 | VV UNA | - | Jong FC Twente | 3:1 |  |
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| 28. Round | 27/03/2017 | Jong FC Twente | - | Sparta Rotterdam (J) | 0:3 |
Netherlands