| 2. Round | 24/01/2010 | Indios Juárez | - | Santos Laguna | 0:0 | ||||
| 3. Round | 31/01/2010 | Santos Laguna | - | Atlas Guadalajara | 1:2 | ||||
| 4. Round | 06/02/2010 | Cruz Azul | - | Santos Laguna | 0:2 | ||||
| 5. Round | 13/02/2010 | Santos Laguna | - | Deportivo Toluca | 1:0 | ||||
| 6. Round | 19/02/2010 | UANL Tigres | - | Santos Laguna | 1:2 |  |
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| 7. Round | 21/02/2010 | Santos Laguna | - | Estudiantes Tecos | 3:3 | ||||
| 8. Round | 27/02/2010 | Gallos Blancos (old) | - | Santos Laguna | 1:1 | ||||
| 9. Round | 07/03/2010 | Santos Laguna | - | Atlante | 4:2 | ||||
| 10. Round | 14/03/2010 | CF Pachuca | - | Santos Laguna | 0:3 | ||||
| 11. Round | 21/03/2010 | Santos Laguna | - | Puebla FC | 2:1 | ||||
| 12. Round | 28/03/2010 | Deportivo Guadalajara | - | Santos Laguna | 6:2 |  |
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| 15. Round | 15/04/2010 | Santos Laguna | - | CF Monterrey | 1:1 | ||||
| 16. Round | 20/04/2010 | Santos Laguna | - | Club San Luis | 2:1 | ||||
| 17. Round | 25/04/2010 | CF América | - | Santos Laguna | 1:0 | |
Uruguay