| 18. Round | 27/01/2001 | Go Ahead Eagles | - | BV Veendam | 3:1 |  |
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| 19. Round | 07/02/2001 | VVV | - | Go Ahead Eagles | 0:2 | ||||
| 20. Round | 10/02/2001 | Go Ahead Eagles | - | Telstar | 1:1 | ||||
| 21. Round | 17/02/2001 | MVV | - | Go Ahead Eagles | 3:1 | |
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| 23. Round | 03/03/2001 | FC Den Bosch | - | Go Ahead Eagles | 1:1 | ||||
| 24. Round | 10/03/2001 | Go Ahead Eagles | - | SBV Excelsior | 4:1 | ||||
| 25. Round | 18/03/2001 | FC Volendam | - | Go Ahead Eagles | 4:2 | |
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| 22. Round | 04/04/2001 | Go Ahead Eagles | - | ADO Den Haag | 0:4 | ||||
| 27. Round | 07/04/2001 | SBV Eindhoven | - | Go Ahead Eagles | 3:1 | ||||
| 29. Round | 21/04/2001 | Go Ahead Eagles | - | HFC Haarlem | 2:1 | ||||
| 30. Round | 28/04/2001 | Dordrecht '90 | - | Go Ahead Eagles | 4:2 | ||||
| 28. Round | 01/05/2001 | SC Cambuur | - | Go Ahead Eagles | 3:0 | ||||
| 33. Round | 19/05/2001 | TOP Oss | - | Go Ahead Eagles | 2:2 | ||||
| 34. Round | 26/05/2001 | Go Ahead Eagles | - | BV Emmen | 1:2 |
Netherlands