| 3. Round | 22/03/2025 | Louisville City FC | - | Loudoun United | 2:0 | ||||
| 5. Round | 06/04/2025 | Sacramento Republic | - | Louisville City FC | 1:1 | ||||
| 9. Round | 11/05/2025 | Louisville City FC | - | Pittsburgh Riverhounds | 0:0 | ||||
| 10. Round | 18/05/2025 | Hartford Athletic | - | Louisville City FC | 1:1 |
USA