| 15. Round | 17/06/2021 | El Nacional | - | Gualaceo SC | 1:1 | ||||
| 17. Round | 25/06/2021 | América de Quito | - | Gualaceo SC | 0:1 |  |
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| 18. Round | 28/06/2021 | Gualaceo SC | - | Cumbayá | 0:1 | ||||
| 19. Round | 03/07/2021 | Atlético Santo Domingo | - | Gualaceo SC | 1:4 |  |
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| 21. Round | 11/07/2021 | Gualaceo SC | - | Guayaquil SC | 2:0 | |
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| 23. Round | 18/07/2021 | Gualaceo SC | - | Atlético Porteño | 4:2 | ||||
| 25. Round | 28/07/2021 | Gualaceo SC | - | América de Quito | 1:0 | ||||
| 26. Round | 04/08/2021 | Liga de Portoviejo | - | Gualaceo SC | 1:1 | ||||
| 27. Round | 11/08/2021 | Gualaceo SC | - | El Nacional | 1:0 | ||||
| 28. Round | 18/08/2021 | Gualaceo SC | - | Independiente Juniors | 2:0 | ||||
| 29. Round | 26/08/2021 | Guayaquil SC | - | Gualaceo SC | 0:2 | ||||
| 32. Round | 15/09/2021 | Gualaceo SC | - | Cumbayá | 3:1 | |
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| 33. Round | 22/09/2021 | América de Quito | - | Gualaceo SC | 1:1 | ||||
| 34. Round | 29/09/2021 | Gualaceo SC | - | Liga de Portoviejo | 1:0 | |
Ecuador