| 25. Round | 06/02/2009 | FC Omniworld | - | Go Ahead Eagles | 1:2 | ||||
| 26. Round | 13/02/2009 | Fortuna Sittard | - | Go Ahead Eagles | 0:1 | ||||
| 27. Round | 20/02/2009 | Go Ahead Eagles | - | SBV Excelsior | 1:1 | ||||
| 28. Round | 28/02/2009 | Helmond Sport | - | Go Ahead Eagles | 1:1 |  |
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| 29. Round | 06/03/2009 | Go Ahead Eagles | - | Telstar | 2:2 | |
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| 30. Round | 13/03/2009 | FC Den Bosch | - | Go Ahead Eagles | 1:0 | ||||
| 31. Round | 20/03/2009 | Go Ahead Eagles | - | FC Emmen | 0:1 | ||||
| 32. Round | 29/03/2009 | AGOVV | - | Go Ahead Eagles | 1:5 | |
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| 33. Round | 03/04/2009 | Go Ahead Eagles | - | MVV | 0:0 | ||||
| 34. Round | 10/04/2009 | FC Eindhoven | - | Go Ahead Eagles | 1:1 | ||||
| 35. Round | 17/04/2009 | HFC Haarlem | - | Go Ahead Eagles | 1:2 | ||||
| 36. Round | 24/04/2009 | Go Ahead Eagles | - | BV Veendam | 2:1 | ||||
| 37. Round | 01/05/2009 | Go Ahead Eagles | - | TOP Oss | 1:0 | |
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| 38. Round | 08/05/2009 | FC Dordrecht | - | Go Ahead Eagles | 3:1 |
Netherlands