| 1. Round | 07/08/2009 | Go Ahead Eagles | - | FC Omniworld | 2:0 | ||||
| 2. Round | 14/08/2009 | RBC Roosendaal | - | Go Ahead Eagles | 1:3 |  |
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| 3. Round | 21/08/2009 | Go Ahead Eagles | - | De Graafschap | 1:0 | |
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| 4. Round | 24/08/2009 | FC Emmen | - | Go Ahead Eagles | 0:1 | ||||
| 5. Round | 28/08/2009 | Go Ahead Eagles | - | Fortuna Sittard | 2:0 | |
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| 6. Round | 04/09/2009 | SBV Excelsior | - | Go Ahead Eagles | 1:1 | |
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| 7. Round | 11/09/2009 | Go Ahead Eagles | - | FC Oss | 3:1 | ||||
| 8. Round | 20/09/2009 | FC Zwolle | - | Go Ahead Eagles | 1:0 | ||||
| 9. Round | 27/09/2009 | Go Ahead Eagles | - | BV Veendam | 3:0 | |
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| 10. Round | 02/10/2009 | FC Den Bosch | - | Go Ahead Eagles | 1:1 | |
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| 11. Round | 09/10/2009 | Go Ahead Eagles | - | AGOVV | 0:0 |
Netherlands