| 1. Round | 16/09/2000 | Ionikos Nikea | - | Athinaikos | 1:0 | ||||
| 2. Round | 23/09/2000 | Athinaikos | - | AEK Athen | 0:3 |  |
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| 3. Round | 30/09/2000 | Panachaiki GE | - | Athinaikos | 3:1 | ||||
| 4. Round | 28/10/2000 | Athinaikos | - | PS Kalamata | 3:2 | ||||
| 5. Round | 04/11/2000 | PAS Giannina | - | Athinaikos | 3:1 | |
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| 6. Round | 10/11/2000 | Athinaikos | - | Panionios GSS | 2:0 | |
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| 9. Round | 03/12/2000 | Athinaikos | - | Paniliakos Pyrgos | 1:1 | ||||
| 10. Round | 09/12/2000 | Panathinaikos | - | Athinaikos | 1:0 | ||||
| 11. Round | 16/12/2000 | Athinaikos | - | PAOK Saloniki | 3:4 | |
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| 12. Round | 02/01/2001 | Skoda Xanthi | - | Athinaikos | 1:0 | |
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| 13. Round | 06/01/2001 | Athinaikos | - | Iraklis Saloniki | 2:0 | ||||
| 14. Round | 14/01/2001 | Athinaikos | - | Olympiakos Piraeus | 1:5 | |
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| 17. Round | 04/02/2001 | AEK Athen | - | Athinaikos | 2:1 | ||||
| 18. Round | 10/02/2001 | Athinaikos | - | Panachaiki GE | 3:1 | ||||
| 19. Round | 18/02/2001 | PS Kalamata | - | Athinaikos | 2:2 | ||||
| 21. Round | 03/03/2001 | Panionios GSS | - | Athinaikos | 1:0 | ||||
| 22. Round | 10/03/2001 | Athinaikos | - | Aris Saloniki | 2:0 | ||||
| 23. Round | 17/03/2001 | Athinaikos | - | OFI Heraklion | 2:3 | ||||
| 24. Round | 31/03/2001 | Paniliakos Pyrgos | - | Athinaikos | 3:0 | |
Greece