| 1. Round | 12/02/2021 | Central Córdoba SdE II | - | Colón de Santa Fe II | 2:0 | ||||
| 2. Round | 19/02/2021 | Colón de Santa Fe II | - | San Lorenzo II | 1:1 | ||||
| 3. Round | 26/02/2021 | Banfield II | - | Colón de Santa Fe II | 0:0 | ||||
| 4. Round | 05/03/2021 | Colón de Santa Fe II | - | Aldosivi II | 0:1 | ||||
| 5. Round | 12/03/2021 | Estudiantes II | - | Colón de Santa Fe II | 1:1 | ||||
| 6. Round | 19/03/2021 | Colón de Santa Fe II | - | Rosario Central II | 1:0 |  |
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| 7. Round | 29/03/2021 | Platense II | - | Colón de Santa Fe II | 2:2 | |
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| 8. Round | 02/04/2021 | Colón de Santa Fe II | - | Argentinos Juniors II | 0:1 | ||||
| 9. Round | 09/04/2021 | River Plate II | - | Colón de Santa Fe II | 7:0 | ||||
| 10. Round | 16/04/2021 | Colón de Santa Fe II | - | Godoy Cruz II | 1:2 | |
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| 11. Round | 23/04/2021 | Racing Club II | - | Colón de Santa Fe II | 0:1 | ||||
| 12. Round | 30/04/2021 | Colón de Santa Fe II | - | Arsenal de Sarandí II | 2:0 | |
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| 13. Round | 07/05/2021 | Colón de Santa Fe II | - | Unión de Santa Fe II | 0:1 |
Argentina