| 1. Round | 17/09/2000 | OFI Heraklion | - | Aris Saloniki | 2:3 | ||||
| 2. Round | 23/09/2000 | OFI Heraklion | - | Olympiakos Piraeus | 1:2 |  |
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| 3. Round | 30/09/2000 | Paniliakos Pyrgos | - | OFI Heraklion | 0:0 | |
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| 4. Round | 28/10/2000 | OFI Heraklion | - | Panathinaikos | 0:0 | ||||
| 10. Round | 08/12/2000 | Ionikos Nikea | - | OFI Heraklion | 1:0 | ||||
| 11. Round | 17/12/2000 | OFI Heraklion | - | AEK Athen | 3:3 | ||||
| 12. Round | 02/01/2001 | Panachaiki GE | - | OFI Heraklion | 2:1 | ||||
| 13. Round | 06/01/2001 | OFI Heraklion | - | PS Kalamata | 1:1 | ||||
| 17. Round | 02/02/2001 | Olympiakos Piraeus | - | OFI Heraklion | 5:0 | ||||
| 18. Round | 10/02/2001 | OFI Heraklion | - | Paniliakos Pyrgos | 2:2 | ||||
| 20. Round | 24/02/2001 | OFI Heraklion | - | PAOK Saloniki | 0:0 | ||||
| 21. Round | 03/03/2001 | Skoda Xanthi | - | OFI Heraklion | 3:2 | ||||
| 23. Round | 17/03/2001 | Athinaikos | - | OFI Heraklion | 2:3 | ||||
| 24. Round | 31/03/2001 | OFI Heraklion | - | Ethnikos Asteras | 2:2 | |
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| 27. Round | 28/04/2001 | OFI Heraklion | - | Panachaiki GE | 0:1 |
Greece