| 1. Round | 25/07/2021 | FC Fastav Zlín | - | Slavia Praha | 0:1 | ||||
| 2. Round | 01/08/2021 | Baník Ostrava | - | FC Fastav Zlín | 5:1 | ||||
| 3. Round | 07/08/2021 | FC Fastav Zlín | - | FK Teplice | 3:0 |  |
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| 4. Round | 14/08/2021 | MFK Karviná | - | FC Fastav Zlín | 2:3 | |
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| 5. Round | 21/08/2021 | FC Fastav Zlín | - | Sigma Olomouc | 1:4 | |
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| 6. Round | 28/08/2021 | Slovan Liberec | - | FC Fastav Zlín | 0:1 | ||||
| 7. Round | 11/09/2021 | FC Fastav Zlín | - | Bohemians Praha 1905 | 1:3 | ||||
| 11. Round | 17/10/2021 | FC Fastav Zlín | - | FC Hradec Králové | 2:3 | ||||
| 12. Round | 23/10/2021 | FK Pardubice | - | FC Fastav Zlín | 0:0 | ||||
| 13. Round | 30/10/2021 | FC Fastav Zlín | - | Dynamo České Budějovice | 2:0 | ||||
| 14. Round | 07/11/2021 | FC Fastav Zlín | - | FK Jablonec | 0:0 | ||||
| 15. Round | 20/11/2021 | FK Mladá Boleslav | - | FC Fastav Zlín | 1:0 | ||||
| 16. Round | 27/11/2021 | FC Fastav Zlín | - | Baník Ostrava | 2:2 | ||||
| 17. Round | 04/12/2021 | FK Teplice | - | FC Fastav Zlín | 4:1 | ||||
| 18. Round | 11/12/2021 | FC Fastav Zlín | - | MFK Karviná | 2:1 | ||||
| 19. Round | 19/12/2021 | Sigma Olomouc | - | FC Fastav Zlín | 1:1 |
Slovakia