| 1. Round | 01/08/2009 | Willem II | - | Vitesse | 3:1 | ||||
| 2. Round | 09/08/2009 | FC Utrecht | - | Willem II | 1:0 | ||||
| 8. Round | 26/09/2009 | PSV Eindhoven | - | Willem II | 3:1 | ||||
| 9. Round | 03/10/2009 | Willem II | - | NEC Nijmegen | 1:1 | ||||
| 10. Round | 17/10/2009 | AFC Ajax | - | Willem II | 4:0 | ||||
| 11. Round | 25/10/2009 | Willem II | - | sc Heerenveen | 4:1 | ||||
| 12. Round | 01/11/2009 | RKC Waalwijk | - | Willem II | 0:1 | ||||
| 13. Round | 08/11/2009 | NAC Breda | - | Willem II | 4:0 | ||||
| 14. Round | 22/11/2009 | Willem II | - | ADO Den Haag | 1:1 |  |
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| 15. Round | 28/11/2009 | Willem II | - | FC Twente | 1:3 | ||||
| 19. Round | 23/01/2010 | sc Heerenveen | - | Willem II | 4:2 |  |
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| 23. Round | 13/02/2010 | ADO Den Haag | - | Willem II | 3:0 | ||||
| 25. Round | 27/02/2010 | Willem II | - | VVV-Venlo | 2:1 | ||||
| 26. Round | 06/03/2010 | Heracles Almelo | - | Willem II | 3:2 | ||||
| 27. Round | 14/03/2010 | Willem II | - | Sparta Rotterdam | 3:1 | ||||
| 29. Round | 27/03/2010 | Vitesse | - | Willem II | 2:1 | ||||
| 31. Round | 11/04/2010 | FC Groningen | - | Willem II | 4:0 | ||||
| 32. Round | 14/04/2010 | Willem II | - | AFC Ajax | 0:2 | ||||
| 33. Round | 18/04/2010 | Willem II | - | AZ Alkmaar | 0:3 | ||||
| 34. Round | 02/05/2010 | Roda JC Kerkrade | - | Willem II | 3:2 |  |
Netherlands