| 17. Round | 19/02/2001 | Viktoria Plzeň | - | Slavia Praha | 1:3 | ||||
| 18. Round | 04/03/2001 | Viktoria Plzeň | - | 1. FC Synot Stare Mesto | 1:0 |  |
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| 19. Round | 11/03/2001 | Chmel Blsany | - | Viktoria Plzeň | 2:1 |  |
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| 20. Round | 18/03/2001 | CU Bohemians Praha | - | Viktoria Plzeň | 3:1 | ||||
| 21. Round | 01/04/2001 | Viktoria Plzeň | - | Slovan Liberec | 1:0 | ||||
| 22. Round | 05/04/2001 | FK Drnovice | - | Viktoria Plzeň | 3:1 | ||||
| 23. Round | 08/04/2001 | Baník Ostrava | - | Viktoria Plzeň | 3:0 | ||||
| 24. Round | 16/04/2001 | Viktoria Plzeň | - | AC Sparta Praha | 2:3 | ||||
| 25. Round | 21/04/2001 | FK Teplice | - | Viktoria Plzeň | 5:1 | ||||
| 26. Round | 29/04/2001 | Viktoria Plzeň | - | Sigma Olomouc | 1:1 | ||||
| 27. Round | 06/05/2001 | FC Marila Příbram | - | Viktoria Plzeň | 3:1 | ||||
| 28. Round | 13/05/2001 | Viktoria Plzeň | - | FC Stavo Artikel Brno | 1:1 | |
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| 29. Round | 18/05/2001 | Viktoria Žižkov | - | Viktoria Plzeň | 3:3 | ||||
| 30. Round | 25/05/2001 | Viktoria Plzeň | - | FK Jablonec 97 | 2:1 | |
Czech Republic