| 1. Round | 08/08/2020 | MFK Zemplín Michalovce | - | Spartak Trnava | 0:2 |  |
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| 2. Round | 11/08/2020 | Spartak Trnava | - | MFK Ružomberok | 3:1 | ||||
| 4. Round | 22/08/2020 | FC DAC 1904 | - | Spartak Trnava | 2:1 | ||||
| 5. Round | 29/08/2020 | Spartak Trnava | - | ŠKF Sereď | 0:2 | ||||
| 6. Round | 12/09/2020 | Slovan Bratislava | - | Spartak Trnava | 2:0 | |
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| 7. Round | 19/09/2020 | Spartak Trnava | - | Zlaté Moravce | 1:0 | ||||
| 8. Round | 26/09/2020 | FK Senica | - | Spartak Trnava | 0:2 | ||||
| 9. Round | 03/10/2020 | Spartak Trnava | - | FC Nitra | 0:2 | ||||
| 10. Round | 18/10/2020 | MŠK Žilina | - | Spartak Trnava | 2:1 |  |
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| 13. Round | 07/11/2020 | MFK Ružomberok | - | Spartak Trnava | 0:1 | ||||
| 14. Round | 21/11/2020 | FK Pohronie | - | Spartak Trnava | 3:3 | |
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| 15. Round | 28/11/2020 | Spartak Trnava | - | FC DAC 1904 | 0:2 | ||||
| 16. Round | 05/12/2020 | ŠKF Sereď | - | Spartak Trnava | 2:6 | ||||
| 17. Round | 13/12/2020 | Spartak Trnava | - | Slovan Bratislava | 0:3 | |
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| 19. Round | 13/02/2021 | Spartak Trnava | - | FK Senica | 1:0 | |
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| 20. Round | 20/02/2021 | FC Nitra | - | Spartak Trnava | 0:1 | ||||
| 21. Round | 27/02/2021 | Spartak Trnava | - | MŠK Žilina | 2:1 | |
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| 22. Round | 06/03/2021 | AS Trenčín | - | Spartak Trnava | 2:0 |
Slovakia