| 13. Round | 23/11/2024 | Fortuna Sittard | - | FC Twente | 1:2 | ||||
| 14. Round | 01/12/2024 | FC Twente | - | Go Ahead Eagles | 3:2 | ||||
| 15. Round | 06/12/2024 | PSV Eindhoven | - | FC Twente | 6:1 | ||||
| 16. Round | 15/12/2024 | FC Twente | - | FC Groningen | 2:0 |  |
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| 17. Round | 21/12/2024 | AZ Alkmaar | - | FC Twente | 1:0 | ||||
| 18. Round | 12/01/2025 | FC Twente | - | Willem II | 6:2 |  |
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| 19. Round | 19/01/2025 | NAC Breda | - | FC Twente | 2:1 | ||||
| 21. Round | 02/02/2025 | Go Ahead Eagles | - | FC Twente | 2:2 | ||||
| 22. Round | 09/02/2025 | sc Heerenveen | - | FC Twente | 3:3 | ||||
| 23. Round | 16/02/2025 | FC Twente | - | RKC Waalwijk | 2:0 | ||||
| 20. Round | 23/02/2025 | FC Twente | - | NEC Nijmegen | 2:0 | |
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| 24. Round | 01/03/2025 | FC Groningen | - | FC Twente | 1:1 | ||||
| 25. Round | 09/03/2025 | FC Twente | - | Almere City FC | 1:0 | ||||
| 27. Round | 30/03/2025 | Heracles Almelo | - | FC Twente | 2:1 | ||||
| 28. Round | 05/04/2025 | FC Twente | - | Fortuna Sittard | 1:1 | |
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| 29. Round | 13/04/2025 | PEC Zwolle | - | FC Twente | 1:1 | ||||
| 30. Round | 24/04/2025 | FC Twente | - | PSV Eindhoven | 1:3 |
Czech Republic