| 1. Round | 19/08/2000 | MVV | - | FC Den Bosch | 1:4 | ||||
| 2. Round | 26/08/2000 | SBV Excelsior | - | MVV | 5:1 | ||||
| 3. Round | 04/09/2000 | MVV | - | FC Volendam | 1:0 | ||||
| 4. Round | 09/09/2000 | SC Cambuur | - | MVV | 0:0 | ||||
| 5. Round | 16/09/2000 | MVV | - | BV Emmen | 0:0 | ||||
| 6. Round | 25/09/2000 | Dordrecht '90 | - | MVV | 3:2 | ||||
| 7. Round | 30/09/2000 | MVV | - | HFC Haarlem | 2:1 |  |
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| 8. Round | 14/10/2000 | Go Ahead Eagles | - | MVV | 1:0 | |
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| 9. Round | 23/10/2000 | MVV | - | Helmond Sport | 3:0 | ||||
| 10. Round | 28/10/2000 | Heracles Almelo | - | MVV | 1:0 | |
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| 12. Round | 11/11/2000 | FC Zwolle | - | MVV | 4:1 | |
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| 13. Round | 18/11/2000 | MVV | - | BV Veendam | 3:0 | ||||
| 14. Round | 25/11/2000 | VVV | - | MVV | 2:0 | ||||
| 15. Round | 02/12/2000 | MVV | - | Telstar | 1:2 | |
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| 17. Round | 16/12/2000 | TOP Oss | - | MVV | 1:0 | ||||
| 19. Round | 03/02/2001 | MVV | - | SBV Excelsior | 0:2 | ||||
| 25. Round | 17/03/2001 | MVV | - | Dordrecht '90 | 1:3 | ||||
| 34. Round | 26/05/2001 | MVV | - | Heracles Almelo | 5:1 |
Netherlands