| 2. Round | 26/08/2000 | Go Ahead Eagles | - | VVV | 2:1 | ||||
| 3. Round | 04/09/2000 | Telstar | - | Go Ahead Eagles | 2:0 | ||||
| 5. Round | 16/09/2000 | ADO Den Haag | - | Go Ahead Eagles | 3:5 | ||||
| 6. Round | 26/09/2000 | Go Ahead Eagles | - | FC Volendam | 2:2 | ||||
| 7. Round | 02/10/2000 | SBV Excelsior | - | Go Ahead Eagles | 4:1 | ||||
| 8. Round | 14/10/2000 | Go Ahead Eagles | - | MVV | 1:0 | ||||
| 9. Round | 25/10/2000 | Go Ahead Eagles | - | TOP Oss | 3:0 | ||||
| 10. Round | 28/10/2000 | BV Emmen | - | Go Ahead Eagles | 2:0 | ||||
| 12. Round | 11/11/2000 | HFC Haarlem | - | Go Ahead Eagles | 2:4 | ||||
| 13. Round | 18/11/2000 | Go Ahead Eagles | - | Dordrecht '90 | 2:2 | ||||
| 15. Round | 02/12/2000 | Go Ahead Eagles | - | Heracles Almelo | 5:0 | ||||
| 18. Round | 27/01/2001 | Go Ahead Eagles | - | BV Veendam | 3:1 | ||||
| 19. Round | 07/02/2001 | VVV | - | Go Ahead Eagles | 0:2 | ||||
| 24. Round | 10/03/2001 | Go Ahead Eagles | - | SBV Excelsior | 4:1 | ||||
| 28. Round | 01/05/2001 | SC Cambuur | - | Go Ahead Eagles | 3:0 | ||||
| 31. Round | 05/05/2001 | Go Ahead Eagles | - | Helmond Sport | 2:0 | ||||
| 33. Round | 19/05/2001 | TOP Oss | - | Go Ahead Eagles | 2:2 |  |
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| 34. Round | 26/05/2001 | Go Ahead Eagles | - | BV Emmen | 1:2 |
Netherlands