| 19. Round | 19/01/2025 | NAC Breda | - | FC Twente | 2:1 | ||||
| 21. Round | 02/02/2025 | Go Ahead Eagles | - | FC Twente | 2:2 |  |
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| 23. Round | 16/02/2025 | FC Twente | - | RKC Waalwijk | 2:0 | ||||
| 20. Round | 23/02/2025 | FC Twente | - | NEC Nijmegen | 2:0 | ||||
| 24. Round | 01/03/2025 | FC Groningen | - | FC Twente | 1:1 | ||||
| 25. Round | 09/03/2025 | FC Twente | - | Almere City FC | 1:0 | ||||
| 26. Round | 16/03/2025 | FC Twente | - | Feyenoord | 2:6 | ||||
| 27. Round | 30/03/2025 | Heracles Almelo | - | FC Twente | 2:1 | ||||
| 28. Round | 05/04/2025 | FC Twente | - | Fortuna Sittard | 1:1 | ||||
| 29. Round | 13/04/2025 | PEC Zwolle | - | FC Twente | 1:1 | ||||
| 30. Round | 24/04/2025 | FC Twente | - | PSV Eindhoven | 1:3 | |
Netherlands