| 1. Round | 09/03/2025 | Las Vegas Lights FC | - | Tampa Bay Rowdies | 1:0 | ||||
| 2. Round | 16/03/2025 | FC Tulsa | - | Tampa Bay Rowdies | 1:0 | ||||
| 3. Round | 22/03/2025 | Miami FC | - | Tampa Bay Rowdies | 1:2 | ||||
| 5. Round | 05/04/2025 | Charleston Battery | - | Tampa Bay Rowdies | 2:1 | ||||
| 10. Round | 18/05/2025 | Tampa Bay Rowdies | - | Rhode Island FC | 0:3 | ||||
| 11. Round | 25/05/2025 | Lexington SC | - | Tampa Bay Rowdies | 1:1 | ||||
| 12. Round | 08/06/2025 | Louisville City FC | - | Tampa Bay Rowdies | 2:1 |
USA