| 22. Round | 18/01/2010 | Go Ahead Eagles | - | SBV Excelsior | 1:0 | ||||
| 24. Round | 24/01/2010 | AGOVV | - | Go Ahead Eagles | 1:1 |  |
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| 26. Round | 05/02/2010 | Go Ahead Eagles | - | FC Volendam | 0:0 | ||||
| 23. Round | 08/02/2010 | FC Oss | - | Go Ahead Eagles | 0:0 | ||||
| 28. Round | 19/02/2010 | Go Ahead Eagles | - | Telstar | 2:1 | ||||
| 25. Round | 22/02/2010 | Go Ahead Eagles | - | FC Den Bosch | 4:1 | ||||
| 29. Round | 26/02/2010 | MVV | - | Go Ahead Eagles | 0:0 | ||||
| 32. Round | 14/03/2010 | Go Ahead Eagles | - | FC Zwolle | 1:0 | ||||
| 33. Round | 19/03/2010 | Helmond Sport | - | Go Ahead Eagles | 0:1 | |
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| 34. Round | 28/03/2010 | Go Ahead Eagles | - | FC Emmen | 0:2 | ||||
| 35. Round | 04/04/2010 | De Graafschap | - | Go Ahead Eagles | 3:2 | ||||
| 36. Round | 09/04/2010 | Fortuna Sittard | - | Go Ahead Eagles | 2:0 | ||||
| 37. Round | 16/04/2010 | Go Ahead Eagles | - | RBC Roosendaal | 3:1 |
Netherlands