| 1. Round | 31/01/2010 | Club Rubio Ñu | - | Club Olimpia | 1:0 |  |
|||
| 3. Round | 12/02/2010 | Club Rubio Ñu | - | Guaraní | 1:1 | ||||
| 5. Round | 21/02/2010 | Tacuary | - | Club Rubio Ñu | 0:1 | ||||
| 6. Round | 01/03/2010 | Club Rubio Ñu | - | Club Nacional | 4:1 | ||||
| 7. Round | 07/03/2010 | Sportivo Trinidense | - | Club Rubio Ñu | 1:1 | ||||
| 9. Round | 17/03/2010 | 3 de Febrero | - | Club Rubio Ñu | 2:3 | ||||
| 10. Round | 21/03/2010 | Club Rubio Ñu | - | Sport Colombia | 2:1 | |
|||
| 11. Round | 27/03/2010 | Cerro Porteño | - | Club Rubio Ñu | 1:1 | ||||
| 12. Round | 04/04/2010 | Club Olimpia | - | Club Rubio Ñu | 1:1 | ||||
| 14. Round | 14/04/2010 | Guaraní | - | Club Rubio Ñu | 3:2 | ||||
| 16. Round | 25/04/2010 | Club Rubio Ñu | - | Tacuary | 3:1 | ||||
| 20. Round | 16/05/2010 | Club Rubio Ñu | - | 3 de Febrero | 0:2 |
Paraguay