| 1. Round | 09/03/2025 | Charleston Battery | - | Louisville City FC | 1:2 | ||||
| 2. Round | 16/03/2025 | Birmingham Legion | - | Louisville City FC | 1:1 | ||||
| 5. Round | 06/04/2025 | Sacramento Republic | - | Louisville City FC | 1:1 | ||||
| 7. Round | 20/04/2025 | Tampa Bay Rowdies | - | Louisville City FC | 1:2 |  |
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| 8. Round | 02/05/2025 | Louisville City FC | - | Lexington SC | 2:0 |
England