| 20. Round | 27/02/2021 | Southampton FC U23 | - | Manchester United U23 | 1:2 | ||||
| 14. Round | 05/03/2021 | Southampton FC U23 | - | Arsenal FC U23 | 2:2 | ||||
| 18. Round | 08/03/2021 | Blackburn Rovers U23 | - | Southampton FC U23 | 5:1 | ||||
| 23. Round | 09/04/2021 | Brighton & Hove Albion U23 | - | Southampton FC U23 | 3:0 | ||||
| 13. Round | 07/05/2021 | Everton FC U23 | - | Southampton FC U23 | 4:0 |
Ireland