| 14. Round | 20/11/2016 | Jong FC Twente | - | VV UNA | 1:3 | ||||
| 21. Round | 28/01/2017 | Jong FC Twente | - | GVVV | 3:1 | ||||
| 23. Round | 11/02/2017 | Jong FC Twente | - | VV Katwijk | 0:1 | ||||
| 24. Round | 20/02/2017 | AZ Alkmaar (J) | - | Jong FC Twente | 1:0 | ||||
| 20. Round | 24/02/2017 | TEC VV | - | Jong FC Twente | 3:0 | ||||
| 25. Round | 05/03/2017 | VV UNA | - | Jong FC Twente | 3:1 |  |
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| 30. Round | 08/04/2017 | Jong FC Twente | - | Kozakken Boys | 0:3 | ||||
| 31. Round | 22/04/2017 | Excelsior Maassluis | - | Jong FC Twente | 1:1 | ||||
| 32. Round | 29/04/2017 | Jong FC Twente | - | De Treffers | 4:1 | ||||
| 33. Round | 06/05/2017 | Jong FC Twente | - | FC Lienden | 0:1 | ||||
| 34. Round | 13/05/2017 | Koninklijke HFC | - | Jong FC Twente | 0:1 | |
Netherlands