| 3. Round | 31/08/2024 | BVV Barendrecht | - | ACV Assen | 2:0 | ||||
| 4. Round | 07/09/2024 | ACV Assen | - | SV Spakenburg | 1:2 | ||||
| 7. Round | 28/09/2024 | SVV Scheveningen | - | ACV Assen | 1:2 | ||||
| 8. Round | 05/10/2024 | ACV Assen | - | Quick Boys | 1:2 | ||||
| 9. Round | 12/10/2024 | VV Noordwijk | - | ACV Assen | 1:2 | ||||
| 10. Round | 19/10/2024 | ACV Assen | - | Jong Almere City FC | 3:3 | ||||
| 11. Round | 26/10/2024 | GVVV | - | ACV Assen | 1:2 | ||||
| 12. Round | 09/11/2024 | ACV Assen | - | RKAV Volendam | 1:2 | ||||
| 13. Round | 16/11/2024 | HHC Hardenberg | - | ACV Assen | 3:0 | ||||
| 14. Round | 23/11/2024 | ACV Assen | - | Excelsior Maassluis | 0:1 | ||||
| 18. Round | 11/01/2025 | ACV Assen | - | Amsterdamsche FC | 3:2 | ||||
| 19. Round | 18/01/2025 | Koninklijke HFC | - | ACV Assen | 0:1 | ||||
| 20. Round | 25/01/2025 | ACV Assen | - | BVV Barendrecht | 0:1 | ||||
| 27. Round | 22/03/2025 | Jong Almere City FC | - | ACV Assen | 6:0 | ||||
| 28. Round | 05/04/2025 | ACV Assen | - | GVVV | 2:0 | ||||
| 31. Round | 03/05/2025 | Excelsior Maassluis | - | ACV Assen | 1:1 |
Netherlands