| 1. Round | 01/10/2009 | FC Twente [Women] | - | ADO Den Haag [Women] | 0:2 | ||||
| 6. Round | 26/11/2009 | ADO Den Haag [Women] | - | FC Twente [Women] | 1:3 | ||||
| 8. Round | 21/01/2010 | FC Twente [Women] | - | AZ Alkmaar [Women] | 0:0 |  |
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| 10. Round | 25/01/2010 | FC Twente [Women] | - | sc Heerenveen [Women] | 1:1 | ||||
| 13. Round | 28/01/2010 | AZ Alkmaar [Women] | - | FC Twente [Women] | 2:0 | ||||
| 12. Round | 18/02/2010 | Willem II [Women] | - | FC Twente [Women] | 3:4 | |
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| 15. Round | 18/03/2010 | sc Heerenveen [Women] | - | FC Twente [Women] | 1:1 | ||||
| 11. Round | 14/04/2010 | FC Twente [Women] | - | ADO Den Haag [Women] | 0:1 | ||||
| 17. Round | 29/04/2010 | FC Twente [Women] | - | Willem II [Women] | 2:2 | ||||
| 19. Round | 12/05/2010 | FC Utrecht [Women] | - | FC Twente [Women] | 1:1 | ||||
| 20. Round | 19/05/2010 | FC Twente [Women] | - | sc Heerenveen [Women] | 3:3 |
Netherlands