| 8. Round | 03/10/2016 | Sparta Rotterdam (J) | - | Jong FC Twente | 4:2 | ||||
| 12. Round | 05/11/2016 | Kozakken Boys | - | Jong FC Twente | 2:0 | ||||
| 14. Round | 20/11/2016 | Jong FC Twente | - | VV UNA | 1:3 | ||||
| 20. Round | 24/02/2017 | TEC VV | - | Jong FC Twente | 3:0 | ||||
| 25. Round | 05/03/2017 | VV UNA | - | Jong FC Twente | 3:1 | ||||
| 26. Round | 11/03/2017 | Jong FC Twente | - | SV Spakenburg | 1:1 | ||||
| 27. Round | 19/03/2017 | VV Sint Bavo | - | Jong FC Twente | 2:1 | ||||
| 28. Round | 27/03/2017 | Jong FC Twente | - | Sparta Rotterdam (J) | 0:3 |  |
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| 29. Round | 01/04/2017 | HHC Hardenberg | - | Jong FC Twente | 1:2 | ||||
| 30. Round | 08/04/2017 | Jong FC Twente | - | Kozakken Boys | 0:3 | ||||
| 31. Round | 22/04/2017 | Excelsior Maassluis | - | Jong FC Twente | 1:1 | ||||
| 32. Round | 29/04/2017 | Jong FC Twente | - | De Treffers | 4:1 |
Netherlands