| 2. Round | 12/08/2016 | Almere City FC | - | Jong Ajax | 1:4 | ||||
| 3. Round | 19/08/2016 | Jong Ajax | - | FC Den Bosch | 5:2 | ||||
| 4. Round | 22/08/2016 | Jong Ajax | - | Fortuna Sittard | 2:1 | ||||
| 5. Round | 26/08/2016 | RKC Waalwijk | - | Jong Ajax | 3:2 | ||||
| 7. Round | 16/09/2016 | MVV | - | Jong Ajax | 1:0 | ||||
| 8. Round | 26/09/2016 | Jong Ajax | - | FC Oss | 7:0 |  |
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| 9. Round | 30/09/2016 | FC Eindhoven | - | Jong Ajax | 2:1 | ||||
| 10. Round | 17/10/2016 | Jong Ajax | - | De Graafschap | 4:2 | ||||
| 11. Round | 21/10/2016 | FC Volendam | - | Jong Ajax | 1:1 | ||||
| 12. Round | 31/10/2016 | Jong Ajax | - | VVV-Venlo | 0:4 | ||||
| 13. Round | 04/11/2016 | Jong Ajax | - | NAC Breda | 2:1 |  |
Netherlands