| 7. Round | 11/09/2021 | MFK Zemplín Michalovce | - | Spartak Trnava | 1:2 | ||||
| 8. Round | 17/09/2021 | Spartak Trnava | - | ŠKF Sereď | 2:0 | ||||
| 9. Round | 26/09/2021 | FC DAC 1904 | - | Spartak Trnava | 0:3 | ||||
| 12. Round | 23/10/2021 | FK Pohronie | - | Spartak Trnava | 0:2 | ||||
| 14. Round | 07/11/2021 | Tatran Liptovský Mikuláš | - | Spartak Trnava | 0:2 | ||||
| 16. Round | 28/11/2021 | Spartak Trnava | - | MFK Ružomberok | 0:3 |  |
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| 18. Round | 10/12/2021 | Spartak Trnava | - | MFK Zemplín Michalovce | 2:0 |
USA