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| 25/09/2013 | 19:00 | KV Oostende | - | Royal Géants Athois | 5:1 | |
| 19:00 | KV Mechelen | - | RBD Borinage | 5:1 (1:0) | ||
| 19:00 | KRC Genk | - | AFC Tubize | 3:1 (0:1) | ||
| 19:00 | KVC Westerlo | - | RAEC Mons | 4:3 (1:0, 1:1, 1:1) pso | ||
| 19:00 | RSC Anderlecht | - | KAS Eupen | 7:0 (4:0) | ||
| 19:00 | KSV Roeselare | - | Waasland-Beveren | 1:5 (1:4) | ||
| 19:00 | ASV Geel | - | Sporting Lokeren | 0:1 (0:0) | ||
| 19:00 | Tempo Overijse | - | KV Kortrijk | 0:1 (0:0) | ||
| 19:00 | CS Visé | - | Oud-Heverlee Leuven | 0:2 (0:0) | ||
| 19:00 | KFC VW Hamme | - | SV Zulte Waregem | 1:2 (1:0) | ||
| 19:00 | Sporting Charleroi | - | KVK Westhoek | 1:0 (1:0) | ||
| 19:00 | Wolvertem SC | - | Cercle Brugge | 0:3 (0:0) | ||
| 19:30 | Lommel United | - | Lierse SK | 2:3 (1:1) | ||
| 19:30 | KAA Gent | - | KFC Oosterzonen | 4:0 | ||
| 19:30 | White Star Bruxelles | - | Standard Liège | 0:4 (0:3) | ||
| 19:45 | KSV Oudenaarde | - | Club Brugge KV | 0:1 (0:0) |
