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| 1st(1st leg) | Bolívar | - | Oriente Petrolero | 1:1 (0:0) | ||
| 2nd (2nd leg) | Oriente Petrolero | - | Bolívar | 0:0 (0:0) | ||
| 1st(1st leg): 20/09/2009 20:30 | 2nd (2nd leg): 24/09/2009 01:30 | Winner: Oriente Petrolero | ||||
| 1st(1st leg) | Real Potosí | - | Blooming | 0:0 (0:0) | ||
| 2nd (2nd leg) | Blooming | - | Real Potosí | 1:0 (1:0) | ||
| 1st(1st leg): 20/09/2009 20:30 | 2nd (2nd leg): 24/09/2009 23:00 | Winner: Blooming | ||||
| 1st(1st leg) | Universitario de Sucre | - | The Strongest | 2:2 (0:2) | ||
| 2nd (2nd leg) | The Strongest | - | Universitario de Sucre | 2:1 (1:0) | ||
| 1st(1st leg): 20/09/2009 23:00 | 2nd (2nd leg): 25/09/2009 01:00 | Winner: The Strongest | ||||
