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| 1st(1st leg) | El Nacional | - | Barcelona | 1:0 (0:0) | ||
| 2nd (2nd leg) | Barcelona | - | El Nacional | 2:4 (0:0, 0:1) pso | ||
| 1st(1st leg): 25/10/2024 18:15 | 2nd (2nd leg): 02/11/2024 16:00 | Winner: El Nacional | ||||
| 1st(1st leg) | Vinotinto | - | Universidad Católica | 0:2 (0:1) | ||
| 2nd (2nd leg) | Universidad Católica | - | Vinotinto | 5:1 (1:1) | ||
| 1st(1st leg): 26/10/2024 14:00 | 2nd (2nd leg): 01/11/2024 14:00 | Winner: Universidad Católica | ||||
| 1st(1st leg) | Cumbayá | - | Macará | 1:2 (0:2) | ||
| 2nd (2nd leg) | Macará | - | Cumbayá | 3:2 (1:0) | ||
| 1st(1st leg): 26/10/2024 15:00 | 2nd (2nd leg): 01/11/2024 20:00 | Winner: Macará | ||||
| 1st(1st leg) | Delfín SC | - | Orense SC | 2:1 (1:1) | ||
| 2nd (2nd leg) | Orense SC | - | Delfín SC | 3:1 (2:0) | ||
| 1st(1st leg): 26/10/2024 15:00 | 2nd (2nd leg): 02/11/2024 20:15 | Winner: Orense SC | ||||
| 1st(1st leg) | Mushuc Runa | - | Independiente Juniors | 2:1 (2:0) | ||
| 2nd (2nd leg) | Independiente Juniors | - | Mushuc Runa | 3:1 (1:0) | ||
| 1st(1st leg): 26/10/2024 17:15 | 2nd (2nd leg): 02/11/2024 14:00 | Winner: Independiente Juniors | ||||
| 1st(1st leg) | Leones del Norte | - | LDU Quito | 0:3 (0:2) | ||
| 2nd (2nd leg) | LDU Quito | - | Leones del Norte | 1:1 (0:0) | ||
| 1st(1st leg): 26/10/2024 18:00 | 2nd (2nd leg): 01/11/2024 14:00 | Winner: LDU Quito | ||||
| 1st(1st leg) | Imbabura | - | Independiente del Valle | 0:1 (0:1) | ||
| 2nd (2nd leg) | Independiente del Valle | - | Imbabura | 5:0 (4:0) | ||
| 1st(1st leg): 26/10/2024 18:15 | 2nd (2nd leg): 03/11/2024 14:00 | Winner: Independiente del Valle | ||||
| 1st(1st leg) | Vargas Torres | - | Aucas | 1:0 (1:0) | ||
| 2nd (2nd leg) | Aucas | - | Vargas Torres | 7:0 (2:0) | ||
| 1st(1st leg): 27/10/2024 18:15 | 2nd (2nd leg): 03/11/2024 16:00 | Winner: Aucas | ||||
