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| 1st(1st leg) | Go Ahead Eagles | - | SC Cambuur | 2:0 (1:0) | ||
| 2nd (2nd leg) | SC Cambuur | - | Go Ahead Eagles | 1:0 (1:0) | ||
| 1st(1st leg): 06/05/2010 18:00 | 2nd (2nd leg): 09/05/2010 13:30 | Winner: Go Ahead Eagles | ||||
| 1st(1st leg) | FC Eindhoven | - | Willem II | 1:2 (1:0) | ||
| 2nd (2nd leg) | Willem II | - | FC Eindhoven | 1:1 (1:0) | ||
| 1st(1st leg): 06/05/2010 19:00 | 2nd (2nd leg): 09/05/2010 13:30 | Winner: Willem II | ||||
| 1st(1st leg) | FC Zwolle | - | SBV Excelsior | 0:1 (0:1) | ||
| 2nd (2nd leg) | SBV Excelsior | - | FC Zwolle | 4:3 (2:2) | ||
| 1st(1st leg): 06/05/2010 19:00 | 2nd (2nd leg): 09/05/2010 13:30 | Winner: SBV Excelsior | ||||
| 1st(1st leg) | Helmond Sport | - | Sparta Rotterdam | 2:1 (0:0) | ||
| 2nd (2nd leg) | Sparta Rotterdam | - | Helmond Sport | 2:0 (0:0) | ||
| 1st(1st leg): 06/05/2010 20:15 | 2nd (2nd leg): 09/05/2010 15:30 | Winner: Sparta Rotterdam | ||||
