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| 1st(1st leg) | MFK Petržalka | - | Spartak Trnava | 3:2 | ||
| 2nd (2nd leg) | Spartak Trnava | - | MFK Petržalka | 3:0 | ||
| 1st(1st leg): 03/11/2009 12:30 | 2nd (2nd leg): 25/11/2009 15:30 | Winner: Spartak Trnava | ||||
| 1st(1st leg) | FC DAC 1904 | - | FK Bodva Moldava | 3:0 | ||
| 2nd (2nd leg) | FK Bodva Moldava | - | FC DAC 1904 | 1:3 | ||
| 1st(1st leg): 03/11/2009 17:00 | 2nd (2nd leg): 24/11/2009 17:00 | Winner: FC DAC 1904 | ||||
| 1st(1st leg) | Spartak Myjava | - | Slovan Bratislava | 0:3 | ||
| 2nd (2nd leg) | Slovan Bratislava | - | Spartak Myjava | 0:0 | ||
| 1st(1st leg): 04/11/2009 12:30 | 2nd (2nd leg): 25/11/2009 16:30 | Winner: Slovan Bratislava | ||||
| 1st(1st leg) | MFK Košice | - | FK Dukla Banská Bystrica | 0:2 | ||
| 2nd (2nd leg) | FK Dukla Banská Bystrica | - | MFK Košice | 1:0 | ||
| 1st(1st leg): 04/11/2009 15:30 | 2nd (2nd leg): 24/11/2009 15:30 | Winner: FK Dukla Banská Bystrica | ||||
