26. Round
15/10/2021 05:00 FC Kariya FC Kariya - Kochi United Kochi United 2:1 (1:1)
16/10/2021 05:00 Honda FC Honda FC - Matsue City FC Matsue City FC 4:1 (1:1)
16/10/2021 05:00 Suzuka Point Getters Suzuka Point Getters - Nara Club Nara Club 2:1 (1:0)
16/10/2021 05:00 Tokyo Musashino City FC Tokyo Musashino City FC - Honda Lock Honda Lock 2:0 (1:0)
17/10/2021 05:00 Iwaki SC Iwaki SC - Sony Sendai Sony Sendai 0:2 (0:1)
17/10/2021 05:00 MIO Biwako Shiga MIO Biwako Shiga - Verspah Oita Verspah Oita 1:2 (0:1)
17/10/2021 05:00 ReinMeer Aomori ReinMeer Aomori - Veertien Mie Veertien Mie 1:1 (0:0)
17/10/2021 05:00 FC Osaka FC Osaka - FC Tiamo Hirakata FC Tiamo Hirakata 1:1 (1:0)
27. Round
23/10/2021 05:00 Veertien Mie Veertien Mie - Tokyo Musashino City FC Tokyo Musashino City FC -:-
24/10/2021 05:00 Verspah Oita Verspah Oita - Maruyasu Okazaki Maruyasu Okazaki -:-
24/10/2021 05:00 Sony Sendai Sony Sendai - Suzuka Point Getters Suzuka Point Getters -:-
24/10/2021 05:00 Matsue City FC Matsue City FC - MIO Biwako Shiga MIO Biwako Shiga -:-
24/10/2021 05:00 Honda Lock Honda Lock - FC Kariya FC Kariya -:-
24/10/2021 05:00 Nara Club Nara Club - FC Osaka FC Osaka -:-
24/10/2021 05:00 Kochi United Kochi United - Honda FC Honda FC -:-
24/10/2021 05:00 FC Tiamo Hirakata FC Tiamo Hirakata - ReinMeer Aomori ReinMeer Aomori -:-